ketone labeling ethanol Search Results


kod dna polymerase  (Toyobo)
96
Toyobo kod dna polymerase
( a ) FAM70 <t>DNA</t> substrate. The 3′ end of the FAM-labeled strand is the tailing target. P, phosphate group. ( b ) MMLV-RT generated tails of A, C, G, and T. Ten nanomolar FAM70 DNA was incubated with MMLV-RT in the presence of the indicated deoxyribonucleoside triphosphates for 5, 30, and 120 min. Unreacted controls are shown at the top. For some peaks, relative sizes are indicated. ( c ) Tails were removed by blunting. Ten nanomolar FAM70 DNA was incubated with MMLV-RT in the presence of dGTP for 2 h, purified by phenol/chloroform/isoamylalcohol extraction and ethanol precipitation, and then subjected to blunting by <t>KOD</t> <t>polymerase.</t>
Kod Dna Polymerase, supplied by Toyobo, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ketone+labeling+ethanol/KOD+DNA+Polymerase/pmc05288710-15-10-13
Average 96 stars, based on 1 article reviews
kod dna polymerase - by Bioz Stars, 2026-10
96/100 stars
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nnk unlabelled in nonane ethanol  (Cambridge Isotope Laboratories)
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NNK unlabelled 100 µg mL in Nonane Ethanol 9 1
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( a ) FAM70 DNA substrate. The 3′ end of the FAM-labeled strand is the tailing target. P, phosphate group. ( b ) MMLV-RT generated tails of A, C, G, and T. Ten nanomolar FAM70 DNA was incubated with MMLV-RT in the presence of the indicated deoxyribonucleoside triphosphates for 5, 30, and 120 min. Unreacted controls are shown at the top. For some peaks, relative sizes are indicated. ( c ) Tails were removed by blunting. Ten nanomolar FAM70 DNA was incubated with MMLV-RT in the presence of dGTP for 2 h, purified by phenol/chloroform/isoamylalcohol extraction and ethanol precipitation, and then subjected to blunting by KOD polymerase.

Journal: Scientific Reports

Article Title: Efficient N-tailing of blunt DNA ends by Moloney murine leukemia virus reverse transcriptase

doi: 10.1038/srep41769

Figure Lengend Snippet: ( a ) FAM70 DNA substrate. The 3′ end of the FAM-labeled strand is the tailing target. P, phosphate group. ( b ) MMLV-RT generated tails of A, C, G, and T. Ten nanomolar FAM70 DNA was incubated with MMLV-RT in the presence of the indicated deoxyribonucleoside triphosphates for 5, 30, and 120 min. Unreacted controls are shown at the top. For some peaks, relative sizes are indicated. ( c ) Tails were removed by blunting. Ten nanomolar FAM70 DNA was incubated with MMLV-RT in the presence of dGTP for 2 h, purified by phenol/chloroform/isoamylalcohol extraction and ethanol precipitation, and then subjected to blunting by KOD polymerase.

Article Snippet: Blunting of DNA ends was conducted by treating DNA with KOD DNA polymerase (TOYOBO, JAPAN) at 60 °C for 10 min according to the manufacturer’s instructions.

Techniques: Labeling, Generated, Incubation, Purification, Extraction, Ethanol Precipitation